Our Oncology Panel A is the first set of our Oncology Panel. It contains 96 lentiviral expression constructs (LentiSH-Cas9-sgRNA-Puro) that express Cas9 and sgRNAs targeting 89 cancer-associated genes. The backbone confers puromycin resistance and is compatible with both 2nd and 3rd generation lentiviral packaging plasmids, which can be purchased separately.
We believe this panel of DNA constructs could facilitate systematic investigation of the
genetic mechanisms of cancer initiation and progression.
Specifications: The specific genomic coordinates and genes isoforms targeted by each
sgRNAs can be found in this list. The Annotated sequence of the LentiSH-Cas9-sgRNA-
Puro can be downloaded here.